Variable aberrant cDNAs in single diphtheria toxin-resistant human fibroblasts

Amiram Ronen; Miriam Broit; Axel Nohturfft; John Curry; Barry W. Glickman

doi:10.1016/S0027-5107(97)00168-1

Variable aberrant cDNAs in single diphtheria toxin-resistant human fibroblasts

Amiram Ronen^*, Miriam Broit, Axel Nohturfft, John Curry, Barry W. Glickman

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We treated transformed human fibroblasts with diphtheria toxin (DT) and isolated 40 single cells that were toxin resistant but unable to propagate. In 13 of them toxin resistance was associated with the presence of one or more aberrant transcripts of the structural gene for elongation factor 2 (EF-2). cDNA obtained from these transcripts had 164-447 bp-long deletions. Each of these deletions was associated with 2-8 base pairs-long repeats at its breakpoints. Only 10 out of 16 cDNA deletions were associated with presumed exon junctions. A role is suggested for errors in transcription in producing the aberrant transcripts which gave rise to the deletion-bearing cDNA species.

Original language	English
Pages (from-to)	109-115
Number of pages	7
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	379
Issue number	2
DOIs	https://doi.org/10.1016/S0027-5107(97)00168-1
State	Published - 6 Oct 1997

Keywords

Diphtheria toxin
Elongation factor 2
Mutation
Non-propagating cell
mRNA deletions

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10.1016/S0027-5107(97)00168-1

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title = "Variable aberrant cDNAs in single diphtheria toxin-resistant human fibroblasts",

abstract = "We treated transformed human fibroblasts with diphtheria toxin (DT) and isolated 40 single cells that were toxin resistant but unable to propagate. In 13 of them toxin resistance was associated with the presence of one or more aberrant transcripts of the structural gene for elongation factor 2 (EF-2). cDNA obtained from these transcripts had 164-447 bp-long deletions. Each of these deletions was associated with 2-8 base pairs-long repeats at its breakpoints. Only 10 out of 16 cDNA deletions were associated with presumed exon junctions. A role is suggested for errors in transcription in producing the aberrant transcripts which gave rise to the deletion-bearing cDNA species.",

keywords = "Diphtheria toxin, Elongation factor 2, Mutation, Non-propagating cell, mRNA deletions",

author = "Amiram Ronen and Miriam Broit and Axel Nohturfft and John Curry and Glickman, \{Barry W.\}",

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T1 - Variable aberrant cDNAs in single diphtheria toxin-resistant human fibroblasts

AU - Ronen, Amiram

AU - Broit, Miriam

AU - Nohturfft, Axel

AU - Curry, John

AU - Glickman, Barry W.

PY - 1997/10/6

Y1 - 1997/10/6

N2 - We treated transformed human fibroblasts with diphtheria toxin (DT) and isolated 40 single cells that were toxin resistant but unable to propagate. In 13 of them toxin resistance was associated with the presence of one or more aberrant transcripts of the structural gene for elongation factor 2 (EF-2). cDNA obtained from these transcripts had 164-447 bp-long deletions. Each of these deletions was associated with 2-8 base pairs-long repeats at its breakpoints. Only 10 out of 16 cDNA deletions were associated with presumed exon junctions. A role is suggested for errors in transcription in producing the aberrant transcripts which gave rise to the deletion-bearing cDNA species.

AB - We treated transformed human fibroblasts with diphtheria toxin (DT) and isolated 40 single cells that were toxin resistant but unable to propagate. In 13 of them toxin resistance was associated with the presence of one or more aberrant transcripts of the structural gene for elongation factor 2 (EF-2). cDNA obtained from these transcripts had 164-447 bp-long deletions. Each of these deletions was associated with 2-8 base pairs-long repeats at its breakpoints. Only 10 out of 16 cDNA deletions were associated with presumed exon junctions. A role is suggested for errors in transcription in producing the aberrant transcripts which gave rise to the deletion-bearing cDNA species.

KW - Diphtheria toxin

KW - Elongation factor 2

KW - Mutation

KW - Non-propagating cell

KW - mRNA deletions

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JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

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Variable aberrant cDNAs in single diphtheria toxin-resistant human fibroblasts

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