Vav, a GDP/GTP nucleotide exchange factor, interacts with GDIs, proteins that inhibit GDP/GTP dissociation

Maya Groysman; Chen Shifrin Niva Russek; Shulamit Katzav

doi:10.1016/S0014-5793(00)01121-2

Vav, a GDP/GTP nucleotide exchange factor, interacts with GDIs, proteins that inhibit GDP/GTP dissociation

Maya Groysman, Chen Shifrin Niva Russek, Shulamit Katzav^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Vav functions as a specific GDP/GTP nucleotide exchange factor which is regulated by tyrosine phosphorylation in the hematopoietic system. Loss of the amino-terminus sequences of Vav was sufficient to control its transforming potential and its function in T cells. We report here the identification of the hematopoietic GDP dissociation inhibitor protein, Ly-GDI, as a protein that interacts with the amino-terminus of Vav. Further analysis confirmed that Vav and Ly-GDI interact both in in vitro and in in vivo assays. This association is maximal only when the amino region of Vav is intact and requires an intact carboxy-terminus of Ly-GDI. The interaction between Vav and Ly-GDI is not dependent on the tyrosine phosphorylation status of Vav. In addition, Rho-GDI, the highly homologous protein to Ly-GDI, associates with Vav as well. The contribution of the interaction between Vav and GDIs, proteins that are involved in the GDP/GTP exchange processes, to the biological function of Vav is further discussed. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	American English
Pages (from-to)	75-80
Number of pages	6
Journal	FEBS Letters
Volume	467
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(00)01121-2
State	Published - 4 Feb 2000
Externally published	Yes

Bibliographical note

Funding Information:
We are indebted to Dr. M.K. Brenner for critical reading of the manuscript, Dr. J. Camonis for giving us reagents necessary for the yeast two hybrid screens and Mr. Y. Markson for help with the figures. This work was supported by a grant from the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities given to S.K.

Keywords

GDP/GTP exchange
Ly-GDI
Rho-GDI
vav

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10.1016/S0014-5793(00)01121-2

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title = "Vav, a GDP/GTP nucleotide exchange factor, interacts with GDIs, proteins that inhibit GDP/GTP dissociation",

abstract = "Vav functions as a specific GDP/GTP nucleotide exchange factor which is regulated by tyrosine phosphorylation in the hematopoietic system. Loss of the amino-terminus sequences of Vav was sufficient to control its transforming potential and its function in T cells. We report here the identification of the hematopoietic GDP dissociation inhibitor protein, Ly-GDI, as a protein that interacts with the amino-terminus of Vav. Further analysis confirmed that Vav and Ly-GDI interact both in in vitro and in in vivo assays. This association is maximal only when the amino region of Vav is intact and requires an intact carboxy-terminus of Ly-GDI. The interaction between Vav and Ly-GDI is not dependent on the tyrosine phosphorylation status of Vav. In addition, Rho-GDI, the highly homologous protein to Ly-GDI, associates with Vav as well. The contribution of the interaction between Vav and GDIs, proteins that are involved in the GDP/GTP exchange processes, to the biological function of Vav is further discussed. Copyright (C) 2000 Federation of European Biochemical Societies.",

keywords = "GDP/GTP exchange, Ly-GDI, Rho-GDI, vav",

author = "Maya Groysman and Russek, {Chen Shifrin Niva} and Shulamit Katzav",

note = "Funding Information: We are indebted to Dr. M.K. Brenner for critical reading of the manuscript, Dr. J. Camonis for giving us reagents necessary for the yeast two hybrid screens and Mr. Y. Markson for help with the figures. This work was supported by a grant from the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities given to S.K.",

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AU - Groysman, Maya

AU - Russek, Chen Shifrin Niva

AU - Katzav, Shulamit

N1 - Funding Information: We are indebted to Dr. M.K. Brenner for critical reading of the manuscript, Dr. J. Camonis for giving us reagents necessary for the yeast two hybrid screens and Mr. Y. Markson for help with the figures. This work was supported by a grant from the Israel Science Foundation founded by the Israel Academy of Sciences and Humanities given to S.K.

PY - 2000/2/4

Y1 - 2000/2/4

N2 - Vav functions as a specific GDP/GTP nucleotide exchange factor which is regulated by tyrosine phosphorylation in the hematopoietic system. Loss of the amino-terminus sequences of Vav was sufficient to control its transforming potential and its function in T cells. We report here the identification of the hematopoietic GDP dissociation inhibitor protein, Ly-GDI, as a protein that interacts with the amino-terminus of Vav. Further analysis confirmed that Vav and Ly-GDI interact both in in vitro and in in vivo assays. This association is maximal only when the amino region of Vav is intact and requires an intact carboxy-terminus of Ly-GDI. The interaction between Vav and Ly-GDI is not dependent on the tyrosine phosphorylation status of Vav. In addition, Rho-GDI, the highly homologous protein to Ly-GDI, associates with Vav as well. The contribution of the interaction between Vav and GDIs, proteins that are involved in the GDP/GTP exchange processes, to the biological function of Vav is further discussed. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - Vav functions as a specific GDP/GTP nucleotide exchange factor which is regulated by tyrosine phosphorylation in the hematopoietic system. Loss of the amino-terminus sequences of Vav was sufficient to control its transforming potential and its function in T cells. We report here the identification of the hematopoietic GDP dissociation inhibitor protein, Ly-GDI, as a protein that interacts with the amino-terminus of Vav. Further analysis confirmed that Vav and Ly-GDI interact both in in vitro and in in vivo assays. This association is maximal only when the amino region of Vav is intact and requires an intact carboxy-terminus of Ly-GDI. The interaction between Vav and Ly-GDI is not dependent on the tyrosine phosphorylation status of Vav. In addition, Rho-GDI, the highly homologous protein to Ly-GDI, associates with Vav as well. The contribution of the interaction between Vav and GDIs, proteins that are involved in the GDP/GTP exchange processes, to the biological function of Vav is further discussed. Copyright (C) 2000 Federation of European Biochemical Societies.

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ER -

Vav, a GDP/GTP nucleotide exchange factor, interacts with GDIs, proteins that inhibit GDP/GTP dissociation

Abstract

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Keywords

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