Abstract
Human erythrocyte ghosts were incubated with the proteolytic enzyme pronase under isotonic (iso-human erythrocyte ghosts) or hypotonic (hypohuman erythrocyte ghosts) conditions. Gel electrophoresis and electron microscope (freeze-etching) studies revealed that most of the erythrocyte membrane polypeptides were hydrolyzed by pronase under hypotonic conditions. Sendai virus readily agglutinated both pronase-digested iso-human erythrocyte ghosts and hypo-human erythrocyte ghosts but was able to fuse only iso-human erythrocyte ghosts. Iso- and hypo-human erythrocyte ghosts were fused by the non-viral fusogenic agent glyceromonooleate. Freeze-etching studies revealed that during fusion the membranes of pronase-digested human erythrocyte ghosts are intermixed.
| Original language | English |
|---|---|
| Pages (from-to) | 282-294 |
| Number of pages | 13 |
| Journal | Biochimica et Biophysica Acta - Biomembranes |
| Volume | 551 |
| Issue number | 2 |
| DOIs | |
| State | Published - 8 Mar 1979 |
Keywords
- (Erythrocyte ghost
- Membrane fusion
- Pronase digestion
- Sendai virus)