Virulence factors of Escherichia coli isolated from bovine clinical mastitis

T. Kaipainen*, T. Pohjanvirta, N. Y. Shpigel, A. Shwimmer, S. Pyörälä, S. Pelkonen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

Escherichia coli isolates from bovine mastitis were examined for a selection of virulence factors. The strains originated from Finland and Israel, which have differences in the proportion of mastitis caused by E. coli, clinical pictures of coliform mastitis, environmental conditions and herd management. The genes of nine virulence factors were detected by polymerase chain reaction. Presence of K1 and K5 capsules was assessed by use of specific bacteriophages. Serum resistance was tested by a turbidimetric assay. Out of 160 Finnish isolates, 37% had traT, 14% cnf2, 8% cnf1, 11% aer, 9% f17, 8% sfa, 7% pap, 1% afa8D and 1% afa8E. Out of 113 Israeli isolates, 41% had traT, 4% aer, 3% cnf2, 1% cnf1, 1% sfa and 1% f17. Some of the genes were distributed among two major pathotype groups, with either f17 family or sfa, pap and cnf1 as major determinants. Genes for F17a, CS31A, Afa7D and Afa7E were not detected. Altogether 49% of Finnish and 42% of Israeli isolates had at least one virulence gene, but genes other than traT were present in only 24% of Finnish and 5% of Israeli isolates. Serum resistance was more common among Finnish (94/160) than Israeli isolates (19/113). K1 and K5 capsules were not detected.

Original languageAmerican English
Pages (from-to)37-46
Number of pages10
JournalVeterinary Microbiology
Volume85
Issue number1
DOIs
StatePublished - 26 Feb 2002

Bibliographical note

Funding Information:
This work was supported by a grant from Valio Ltd. and the Mercedes Zachariassen Foundation.

Keywords

  • Adhesion
  • Cattle-bacteria
  • Escherichia coli
  • Fimbriae
  • Mastitis
  • Toxin
  • Virulence factor

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