Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes

Ben Spitzer-Rimon, Alon Cna'Ani, Alexander Vainstein

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

9 Scopus citations

Abstract

Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting.

Original languageEnglish
Title of host publicationVirus-Induced Gene Silencing
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages139-148
Number of pages10
ISBN (Print)9781627032773
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume975
ISSN (Print)1064-3745

Keywords

  • Anthocyanin
  • Functional analysis
  • MYB
  • Silencing
  • TRV
  • Virus-aided gene expression (VAGE)
  • Virus-induced gene silencing (VIGS)
  • Volatile

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