@inbook{4490375bba924856b9c4439cef40916e,
title = "Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes",
abstract = "Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting.",
keywords = "Anthocyanin, Functional analysis, MYB, Silencing, TRV, Virus-aided gene expression (VAGE), Virus-induced gene silencing (VIGS), Volatile",
author = "Ben Spitzer-Rimon and Alon Cna'Ani and Alexander Vainstein",
year = "2013",
doi = "10.1007/978-1-62703-278-0_10",
language = "American English",
isbn = "9781627032773",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "139--148",
booktitle = "Virus-Induced Gene Silencing",
}