TY - JOUR
T1 - Whole-cell biodetection of halogenated organic acids
AU - Tauber, Merav
AU - Rosen, Rachel
AU - Belkin, Shimshon
PY - 2001/12/13
Y1 - 2001/12/13
N2 - A whole-cell bacterial sensor system for short-chain halo-organic acids was constructed, using 2-chloropropionic acid (2-CPA) as a model pollutant. An Escherichia coli host was transformed with a moderate-copy plasmid containing a fusion of two foreign genetic elements: (a) a promoter-containing segment of the Pseudomonas DL-DEX (DL-2-haloacid dehalogenase) encoding gene and (b) bioluminescence (luxCDABE) genes of Photorhabdus luminescens. The resulting construct, named MT1, responded to the presence of 2-CPA by dose-dependent light emission, in a highly specific albeit a very insensitive manner. Thus, while the desired concept was successfully demonstrated, further genetic work is needed in order to make such a construct practical for environmental monitoring purposes.
AB - A whole-cell bacterial sensor system for short-chain halo-organic acids was constructed, using 2-chloropropionic acid (2-CPA) as a model pollutant. An Escherichia coli host was transformed with a moderate-copy plasmid containing a fusion of two foreign genetic elements: (a) a promoter-containing segment of the Pseudomonas DL-DEX (DL-2-haloacid dehalogenase) encoding gene and (b) bioluminescence (luxCDABE) genes of Photorhabdus luminescens. The resulting construct, named MT1, responded to the presence of 2-CPA by dose-dependent light emission, in a highly specific albeit a very insensitive manner. Thus, while the desired concept was successfully demonstrated, further genetic work is needed in order to make such a construct practical for environmental monitoring purposes.
KW - Bioluminescence
KW - Escherichia coli
KW - Halogenated organic acids
KW - Microbial biosensors
UR - http://www.scopus.com/inward/record.url?scp=0035856747&partnerID=8YFLogxK
U2 - 10.1016/S0039-9140(01)00492-1
DO - 10.1016/S0039-9140(01)00492-1
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AN - SCOPUS:0035856747
SN - 0039-9140
VL - 55
SP - 959
EP - 964
JO - Talanta
JF - Talanta
IS - 5
ER -