Whole-cell biodetection of halogenated organic acids

Merav Tauber; Rachel Rosen; Shimshon Belkin

doi:10.1016/S0039-9140(01)00492-1

Whole-cell biodetection of halogenated organic acids

Merav Tauber
, Rachel Rosen
, Shimshon Belkin^*

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A whole-cell bacterial sensor system for short-chain halo-organic acids was constructed, using 2-chloropropionic acid (2-CPA) as a model pollutant. An Escherichia coli host was transformed with a moderate-copy plasmid containing a fusion of two foreign genetic elements: (a) a promoter-containing segment of the Pseudomonas DL-DEX (DL-2-haloacid dehalogenase) encoding gene and (b) bioluminescence (luxCDABE) genes of Photorhabdus luminescens. The resulting construct, named MT1, responded to the presence of 2-CPA by dose-dependent light emission, in a highly specific albeit a very insensitive manner. Thus, while the desired concept was successfully demonstrated, further genetic work is needed in order to make such a construct practical for environmental monitoring purposes.

Original language	English
Pages (from-to)	959-964
Number of pages	6
Journal	Talanta
Volume	55
Issue number	5
DOIs	https://doi.org/10.1016/S0039-9140(01)00492-1
State	Published - 13 Dec 2001

Keywords

Bioluminescence
Escherichia coli
Halogenated organic acids
Microbial biosensors

Access to Document

10.1016/S0039-9140(01)00492-1

Cite this

@article{8eefaf4876334d19bd9ba7d403ff0f89,

title = "Whole-cell biodetection of halogenated organic acids",

abstract = "A whole-cell bacterial sensor system for short-chain halo-organic acids was constructed, using 2-chloropropionic acid (2-CPA) as a model pollutant. An Escherichia coli host was transformed with a moderate-copy plasmid containing a fusion of two foreign genetic elements: (a) a promoter-containing segment of the Pseudomonas DL-DEX (DL-2-haloacid dehalogenase) encoding gene and (b) bioluminescence (luxCDABE) genes of Photorhabdus luminescens. The resulting construct, named MT1, responded to the presence of 2-CPA by dose-dependent light emission, in a highly specific albeit a very insensitive manner. Thus, while the desired concept was successfully demonstrated, further genetic work is needed in order to make such a construct practical for environmental monitoring purposes.",

keywords = "Bioluminescence, Escherichia coli, Halogenated organic acids, Microbial biosensors",

author = "Merav Tauber and Rachel Rosen and Shimshon Belkin",

year = "2001",

month = dec,

day = "13",

doi = "10.1016/S0039-9140(01)00492-1",

language = "אנגלית",

volume = "55",

pages = "959--964",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

number = "5",

}

TY - JOUR

T1 - Whole-cell biodetection of halogenated organic acids

AU - Tauber, Merav

AU - Rosen, Rachel

AU - Belkin, Shimshon

PY - 2001/12/13

Y1 - 2001/12/13

N2 - A whole-cell bacterial sensor system for short-chain halo-organic acids was constructed, using 2-chloropropionic acid (2-CPA) as a model pollutant. An Escherichia coli host was transformed with a moderate-copy plasmid containing a fusion of two foreign genetic elements: (a) a promoter-containing segment of the Pseudomonas DL-DEX (DL-2-haloacid dehalogenase) encoding gene and (b) bioluminescence (luxCDABE) genes of Photorhabdus luminescens. The resulting construct, named MT1, responded to the presence of 2-CPA by dose-dependent light emission, in a highly specific albeit a very insensitive manner. Thus, while the desired concept was successfully demonstrated, further genetic work is needed in order to make such a construct practical for environmental monitoring purposes.

AB - A whole-cell bacterial sensor system for short-chain halo-organic acids was constructed, using 2-chloropropionic acid (2-CPA) as a model pollutant. An Escherichia coli host was transformed with a moderate-copy plasmid containing a fusion of two foreign genetic elements: (a) a promoter-containing segment of the Pseudomonas DL-DEX (DL-2-haloacid dehalogenase) encoding gene and (b) bioluminescence (luxCDABE) genes of Photorhabdus luminescens. The resulting construct, named MT1, responded to the presence of 2-CPA by dose-dependent light emission, in a highly specific albeit a very insensitive manner. Thus, while the desired concept was successfully demonstrated, further genetic work is needed in order to make such a construct practical for environmental monitoring purposes.

KW - Bioluminescence

KW - Escherichia coli

KW - Halogenated organic acids

KW - Microbial biosensors

UR - https://www.scopus.com/pages/publications/0035856747

U2 - 10.1016/S0039-9140(01)00492-1

DO - 10.1016/S0039-9140(01)00492-1

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0035856747

SN - 0039-9140

VL - 55

SP - 959

EP - 964

JO - Talanta

JF - Talanta

IS - 5

ER -

Whole-cell biodetection of halogenated organic acids

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this