Wiring of glucose oxidase to carbon matrices via sol-gel derived redox modified silicate

Jenny Gun, Ovadia Lev

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

A new type of porous, composite material is synthesized and used for direct wiring of active enzymes. The material is comprised of a dispersion of graphite powder and redox enzymes incorporated in hybrid, pendant ferrocenyl-, alkylamine- and methyl- modified silicate backbone. Each species in this integrated construction accomplishes a specialized task; the percolating graphite powder provides conductivity, the silicate provides highly crosslinked, rigid backbone, which is used to cage the redox enzymes; ferrocene functional groups are responsible for the signal transduction from the active center of the enzyme to the percolating graphite; amine groups were incorporated for their high affinity to excess negative charges on the surface of glucose oxidase; and finally, the combination of methyl- and amine- groups is advantageous to maintain a control over the thickness of the wetted electroactive section of the electrode. Amperometric sensing of glucose demonstrates the application of this new material.

Original languageAmerican English
Pages (from-to)1933-1938
Number of pages6
JournalAnalytical Letters
Volume29
Issue number11
DOIs
StatePublished - 1996

Bibliographical note

Funding Information:
This research was supported by a grant fiom the ministry of science and the arts of lsrael and the GBF-Gesellschaft Fuer Biotechnologische Forschung, MBH, Braunschweig, Germany. We gratefully acknowledge useful discussions with B. Ginzburg and S. Sampath.

Keywords

  • Composite ceramic-carbon electrodes
  • Enzyme wiring
  • Ferrocenyl modified silicate

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