Xenodiagnosis of Leishmania donovani in BALB/c mice using Phlebotomus orientalis: A new laboratory model

Jovana Sadlova*, Veronika Seblova, Jan Votypka, Alon Warburg, Petr Volf

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Background: In areas endemic for visceral leishmaniasis (VL), the majority of infected hosts remain asymptomatic but potentially infectious to biting sand flies. Their infectiousness for sand fly vectors is crucial for the transmission of the disease and can be quantified only by xenodiagnosis. However, in the case of human hosts, xenodiagnosis can be problematic for ethical and logistic reasons. The BALB/c mouse model described in this paper was designed to enable xenodiagnostic studies on VL hosts circumventing the need for human volunteers, it permits xenodiagnosis using the same individual host repeatedly, over several months. Methods: BALB/c mice were intradermally inoculated in the ear pinnae with Leishmania donovani, primarily metacyclic stages isolated from the thoracic midguts of experimentally-infected Phlebotomus orientalis females. Naïve sand flies were allowed to feed on anaesthetized mice in 1-3-weeks- interval, firstly on the site of inoculation of L. donovani (weeks 2-8 post infection, p.i.), later on the whole body of mice (weeks 9 - 15 p.i.). Infections of sand flies were evaluated microscopically or by PCR analysis. Results: Although infected mice did not show any signs of disease, 19% (N∈=∈876) of the P. orientalis females that fed at the site of inoculation, became infected. The majority of L. donovani-positive females (76%) had heavy infections with their stomodeal valves colonized by attached parasites. Inoculated mouse ears remained infective for sand flies until week 15 p.i. Females feeding on other parts of the body remained negative with exception of two groups feeding on contralateral ears by week 12 p.i. On week 15, however, these two mice returned negative at xenodiagnosis of the contralateral ears. In sacrificed mice, the highest parasite numbers were found in inoculated ears and their draining lymph nodes. Infections were detected also in the spleen, liver, blood and rarely in the contralateral ear. Conclusions: The study showed that BALB/c mice harbored parasites in sufficient numbers to promote heavy infections in P. orientalis and thus comprised a suitable laboratory model for xenodiagnoses of L. donovani. Parasites persisted in the inoculation site and were found transmissible for months to sand flies biting on the same site.

Original languageAmerican English
Article number158
JournalParasites and Vectors
Volume8
Issue number1
DOIs
StatePublished - 14 Mar 2015

Bibliographical note

Funding Information:
We would like to thank to Prof. Teshome Gebre-Michael and Prof. Asrat Hailu, Addis Ababa University, Ethiopia, for kindly providing the progeny of the P. orientalis colony and the L. donovani isolate. This work was supported by Czech Science Foundation [13-07500S], the Bill and Melinda Gates Foundation Global Health Program [OPPGH5336 to A.W.], EDENext [2011–261504 to P.V.] where the paper is cataloged by the EDENext Steering Committee as EDENext280, by EurNegVec COST Action [TD1303] and COST-CZ LD [14076].

Publisher Copyright:
© 2015 Sadlova et al.; licensee BioMed Central.

Keywords

  • Asymptomatic reservoir hosts
  • Leishmania donovani
  • Phlebotomine sand flies
  • Vector-borne diseases
  • Visceral leishmaniasis
  • Xenodiagnosis

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