TY - JOUR
T1 - Yolk sac macrophage progenitors traffic to the embryo during defined stages of development
AU - Stremmel, C.
AU - Schuchert, R.
AU - Wagner, F.
AU - Thaler, R.
AU - Weinberger, T.
AU - Pick, R.
AU - Mass, E.
AU - Ishikawa-Ankerhold, H. C.
AU - Margraf, A.
AU - Hutter, S.
AU - Vagnozzi, R.
AU - Klapproth, S.
AU - Frampton, J.
AU - Yona, S.
AU - Scheiermann, C.
AU - Molkentin, J. D.
AU - Jeschke, U.
AU - Moser, M.
AU - Sperandio, M.
AU - Massberg, S.
AU - Geissmann, F.
AU - Schulz, C.
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Tissue macrophages in many adult organs originate from yolk sac (YS) progenitors, which invade the developing embryo and persist by means of local self-renewal. However, the route and characteristics of YS macrophage trafficking during embryogenesis are incompletely understood. Here we show the early migration dynamics of YS-derived macrophage progenitors in vivo using fate mapping and intravital microscopy. From embryonic day 8.5 (E8.5) CX3CR1+ pre-macrophages are present in the mouse YS where they rapidly proliferate and gain access to the bloodstream to migrate towards the embryo. Trafficking of pre-macrophages and their progenitors from the YS to tissues peaks around E10.5, dramatically decreases towards E12.5 and is no longer evident from E14.5 onwards. Thus, YS progenitors use the vascular system during a restricted time window of embryogenesis to invade the growing fetus. These findings close an important gap in our understanding of the development of the innate immune system.
AB - Tissue macrophages in many adult organs originate from yolk sac (YS) progenitors, which invade the developing embryo and persist by means of local self-renewal. However, the route and characteristics of YS macrophage trafficking during embryogenesis are incompletely understood. Here we show the early migration dynamics of YS-derived macrophage progenitors in vivo using fate mapping and intravital microscopy. From embryonic day 8.5 (E8.5) CX3CR1+ pre-macrophages are present in the mouse YS where they rapidly proliferate and gain access to the bloodstream to migrate towards the embryo. Trafficking of pre-macrophages and their progenitors from the YS to tissues peaks around E10.5, dramatically decreases towards E12.5 and is no longer evident from E14.5 onwards. Thus, YS progenitors use the vascular system during a restricted time window of embryogenesis to invade the growing fetus. These findings close an important gap in our understanding of the development of the innate immune system.
UR - http://www.scopus.com/inward/record.url?scp=85042750501&partnerID=8YFLogxK
U2 - 10.1038/s41467-017-02492-2
DO - 10.1038/s41467-017-02492-2
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C2 - 29311541
AN - SCOPUS:85042750501
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 75
ER -